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http://hdl.handle.net/2289/7354| Title: | Biophysics of Cell-Substrate Interactions Under Shear |
| Authors: | Paddillaya, Neha Mishra, Ashish Pullarkat, Pramod A. +3 Co-Authors |
| Keywords: | focal adhesions stress fibers mechanotransduction shear stress and devices biophysical models adhesion strength |
| Issue Date: | 8-Nov-2019 |
| Publisher: | Frontiers |
| Citation: | Frontiers in Cell and Developmental Biology: Cell Adhesion and Migration,2019, Vol.7, Article No.251 |
| Abstract: | Cells adhere to substrates through mechanosensitive focal adhesion complexes. Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells. Such forces can be applied through shear devices that flow fluid in a controlled manner across cells. The signaling pathways associated with focal adhesions, in particular those that involve integrins and receptor tyrosine kinases, are complex, receiving mechano-chemical feedback from the sensing of substrate stiffness as well as of external forces. This article reviews the signaling processes involved in mechanosensing and mechanotransduction during cell-substrate interactions, describing the role such signaling plays in cancer metastasis. We examine some recent progress in quantifying the strength of these interactions, describing a novel fluid shear device that allows for the visualization of the cell and its sub-cellular structures under a shear flow. We also summarize related results from a biophysical model for cellular de-adhesion induced by applied forces. Quantifying cell-substrate adhesions under shear should aid in the development of mechano-diagnostic techniques for diseases in which cell-adhesion is mis-regulated, such as cancers. |
| Description: | Open Access. |
| URI: | http://hdl.handle.net/2289/7354 |
| ISSN: | 2296-634X (Online) |
| Alternative Location: | https://doi.org/10.3389/fcell.2019.00251 |
| Copyright: | 2019 Paddillaya, Mishra, Kondaiah, Pullarkat, Menon and Gundiah |
| Appears in Collections: | Research Papers (SCM) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2019_Frontiers in Cell and Developmental Biology_Vol.7_Article No.251.pdf Restricted Access | Open Access | 1.3 MB | Adobe PDF | View/Open Request a copy |
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