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http://hdl.handle.net/2289/6496
Title: | Fabrication of low noise borosilicate glass nanopores for single molecule sensing |
Authors: | Bafna, Jayesha A. Soni, G.V. |
Issue Date: | 10-Jun-2016 |
Publisher: | Public Library of Science |
Citation: | PLoS ONE, 2016, Vol. 11, pe-0157399 |
Abstract: | We show low-cost fabrication and characterization of borosilicate glass nanopores for single molecule sensing. Nanopores with diameters of ~100 nm were fabricated in borosilicate glass capillaries using laser assisted glass puller. We further achieve controlled reduction and nanometer-size control in pore diameter by sculpting them under constant electron beam exposure. We successfully fabricate pore diameters down to 6 nm. We next show electrical characterization and low-noise behavior of these borosilicate nanopores and compare their taper geometries. We show, for the first time, a comprehensive characterization of glass nanopore conductance across six-orders of magnitude (1M-1μM) of salt conditions, highlighting the role of buffer conditions. Finally, we demonstrate single molecule sensing capabilities of these devices with real-time translocation experiments of individual λ-DNA molecules. We observe distinct current blockage signatures of linear as well as folded DNA molecules as they undergo voltage-driven translocation through the glass nanopores. We find increased signal to noise for single molecule detection for higher trans-nanopore driving voltages. We propose these nanopores will expand the realm of applications for nanopore platform. |
Description: | Open Access |
URI: | http://hdl.handle.net/2289/6496 |
ISSN: | 1932-6203 1932-6203(Online) |
Alternative Location: | http://dx.doi.org/10.1371/journal.pone.0157399 |
Copyright: | 2016 Bafina, Soni |
Appears in Collections: | Research Papers (SCM) |
Files in This Item:
File | Description | Size | Format | |
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2016_PLOSone_June_0157399.PDF Restricted Access | Open Access | 2.5 MB | Adobe PDF | View/Open Request a copy |
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