Fabrication of low noise borosilicate glass nanopores for single molecule sensing

Bafna, Jayesha A.; Soni, G.V.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/6496

Full metadata record

DC Field	Value	Language
dc.contributor.author	Bafna, Jayesha A.	-
dc.contributor.author	Soni, G.V.	-
dc.date.accessioned	2016-11-28T09:51:11Z	-
dc.date.available	2016-11-28T09:51:11Z	-
dc.date.issued	2016-06-10	-
dc.identifier.citation	PLoS ONE, 2016, Vol. 11, pe-0157399	en_US
dc.identifier.issn	1932-6203	-
dc.identifier.issn	1932-6203(Online)	-
dc.identifier.uri	http://hdl.handle.net/2289/6496	-
dc.description	Open Access	en_US
dc.description.abstract	We show low-cost fabrication and characterization of borosilicate glass nanopores for single molecule sensing. Nanopores with diameters of ~100 nm were fabricated in borosilicate glass capillaries using laser assisted glass puller. We further achieve controlled reduction and nanometer-size control in pore diameter by sculpting them under constant electron beam exposure. We successfully fabricate pore diameters down to 6 nm. We next show electrical characterization and low-noise behavior of these borosilicate nanopores and compare their taper geometries. We show, for the first time, a comprehensive characterization of glass nanopore conductance across six-orders of magnitude (1M-1μM) of salt conditions, highlighting the role of buffer conditions. Finally, we demonstrate single molecule sensing capabilities of these devices with real-time translocation experiments of individual λ-DNA molecules. We observe distinct current blockage signatures of linear as well as folded DNA molecules as they undergo voltage-driven translocation through the glass nanopores. We find increased signal to noise for single molecule detection for higher trans-nanopore driving voltages. We propose these nanopores will expand the realm of applications for nanopore platform.	en_US
dc.language.iso	en	en_US
dc.publisher	Public Library of Science	en_US
dc.relation.uri	http://dx.doi.org/10.1371/journal.pone.0157399	en_US
dc.rights	2016 Bafina, Soni	en_US
dc.title	Fabrication of low noise borosilicate glass nanopores for single molecule sensing	en_US
dc.type	Article	en_US
Appears in Collections:	Research Papers (SCM)

Files in This Item:

File	Description	Size	Format
2016_PLOSone_June_0157399.PDF Restricted Access	Open Access	2.5 MB	Adobe PDF	View/Open Request a copy

Show simple item record