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Title: Temperature driven annealing of perforations in bicellar model membranes
Authors: Nieh, Mu-Ping
Raghunathan, V.A.
Pabst, G.
Harroun, Thad
Nagashima, Kazuomi
Morales, Hannah
Katsaras, J.
Keywords: Solid-State NMR
High-Resolution NMR
Oriented Phospholipid Micelles
Residual Dipolar Couplings
Liquid-Crystalline Phases
Field-Gradient NMR
Transmembrane Domain
Curvature Elasticity
Issue Date: Apr-2011
Publisher: American Chemical Society
Citation: Langmuir, 2011, Vol.27, p4838
Abstract: Bicellar model membranes composed of 1,2-dimyristoylphosphatidylcholine (DMPC) and 1,2-dihexanoylphosphatidylcholine (DHPC), with a DMPC/DHPC molar ratio of 5, and doped with the negatively charged lipid 1,2-dimyristoylphosphatidylglycerol (DMPG), at DMPG/DMPC molar ratios of 0.02 or 0.1, were examined using small angle neutron scattering (SANS), (31)P NMR, and (1)H pulsed field gradient (PFG) diffusion NMR with the goal of understanding temperature effects on the DHPC-dependent perforations in these self-assembled membrane mimetics. Over the temperature range studied via SANS (300-330 K), these bicellar lipid mixtures exhibited a well-ordered lamellar phase. The interlamellar spacing d increased with increasing temperature, in direct contrast to the decrease in d observed upon increasing temperature with otherwise identical lipid mixtures lacking DHPC. (31)P NMR measurements on magnetically aligned bicellar mixtures of identical composition indicated a progressive migration of DHPC from regions of high curvature into planar regions with increasing temperature, and in accord with the "mixed bicelle model" (Triba, M. N.; Warschawski, D. E.; Devaux, P. E. Biophys. J. 2005, 88, 1887-1901). Parallel PFG diffusion NMR measurements of transbilayer water diffusion, where the observed diffusion is dependent on the fractional surface area of lamellar perforations, showed that transbilayer water diffusion decreased with increasing temperature. A model is proposed consistent with the SANS, (31)P NMR, and PFG diffusion NMR data, wherein increasing temperature drives the progressive migration of DHPC out of high-curvature regions, consequently decreasing the fractional volume of lamellar perforations, so that water occupying these perforations redistributes into the interlamellar volume, thereby increasing the interlamellar spacing.
Description: Restricted Access.
ISSN: 0743-7463
1520-5827 (Online)
Alternative Location:
Copyright: 2011 American Chemical Society.
Appears in Collections:Research Papers (SCM)

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