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Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/4084
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dc.contributor.authorNieh, Mu-Ping-
dc.contributor.authorRaghunathan, V.A.-
dc.contributor.authorPabst, G.-
dc.contributor.authorHarroun, Thad-
dc.contributor.authorNagashima, Kazuomi-
dc.contributor.authorMorales, Hannah-
dc.contributor.authorKatsaras, J.-
dc.date.accessioned2011-08-11T07:13:23Z-
dc.date.available2011-08-11T07:13:23Z-
dc.date.issued2011-04-
dc.identifier.citationLangmuir, 2011, Vol.27, p4838en
dc.identifier.issn0743-7463-
dc.identifier.issn1520-5827 (Online)-
dc.identifier.urihttp://hdl.handle.net/2289/4084-
dc.descriptionRestricted Access.en
dc.description.abstractBicellar model membranes composed of 1,2-dimyristoylphosphatidylcholine (DMPC) and 1,2-dihexanoylphosphatidylcholine (DHPC), with a DMPC/DHPC molar ratio of 5, and doped with the negatively charged lipid 1,2-dimyristoylphosphatidylglycerol (DMPG), at DMPG/DMPC molar ratios of 0.02 or 0.1, were examined using small angle neutron scattering (SANS), (31)P NMR, and (1)H pulsed field gradient (PFG) diffusion NMR with the goal of understanding temperature effects on the DHPC-dependent perforations in these self-assembled membrane mimetics. Over the temperature range studied via SANS (300-330 K), these bicellar lipid mixtures exhibited a well-ordered lamellar phase. The interlamellar spacing d increased with increasing temperature, in direct contrast to the decrease in d observed upon increasing temperature with otherwise identical lipid mixtures lacking DHPC. (31)P NMR measurements on magnetically aligned bicellar mixtures of identical composition indicated a progressive migration of DHPC from regions of high curvature into planar regions with increasing temperature, and in accord with the "mixed bicelle model" (Triba, M. N.; Warschawski, D. E.; Devaux, P. E. Biophys. J. 2005, 88, 1887-1901). Parallel PFG diffusion NMR measurements of transbilayer water diffusion, where the observed diffusion is dependent on the fractional surface area of lamellar perforations, showed that transbilayer water diffusion decreased with increasing temperature. A model is proposed consistent with the SANS, (31)P NMR, and PFG diffusion NMR data, wherein increasing temperature drives the progressive migration of DHPC out of high-curvature regions, consequently decreasing the fractional volume of lamellar perforations, so that water occupying these perforations redistributes into the interlamellar volume, thereby increasing the interlamellar spacing.en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.urihttp://dx.doi.org/10.1021/la104750xen
dc.rights2011 American Chemical Society.en
dc.subjectSolid-State NMRen
dc.subjectHigh-Resolution NMRen
dc.subjectOriented Phospholipid Micellesen
dc.subjectResidual Dipolar Couplingsen
dc.subjectNuclear-Magnetic-Resonanceen
dc.subjectLiquid-Crystalline Phasesen
dc.subjectField-Gradient NMRen
dc.subjectLipid-Bilayersen
dc.subjectTransmembrane Domainen
dc.subjectCurvature Elasticityen
dc.titleTemperature driven annealing of perforations in bicellar model membranesen
dc.typeArticleen
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