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http://hdl.handle.net/2289/4038
Title: | The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites |
Authors: | Fernandez, Pablo Pullarkat, Pramod A. |
Keywords: | Atomic-force microscopy nerve growth-factor cell-volume fibroblasts microtubules permeability organization kinetics water |
Issue Date: | 1-Dec-2010 |
Publisher: | Elsevier for Biophysical Society |
Citation: | Biophysical Journal, 2010, Vol.99, p3571 |
Abstract: | We present investigations on volume regulation and beading shape transitions in PC12 neurites conducted using a flow chamber technique By disrupting the cell cytoskeleton with specific drugs we investigate the role of its individual components in the volume regulation response We find that microtubule disruption increases both swelling rate and maximum volume attained but does not affect the ability of the neurite to recover its initial volume In addition investigation of axonal beading also known as pearling instability provides additional clues on the mechanical state of the neurite We conclude that volume recovery is driven by passive diffusion of osmolites and propose that the initial swelling phase is mechanically slowed down by microtubules Our experiments provide a framework to investigate the role of cytoskeletal mechanics in volume homeostasis |
Description: | Restricted Access. Articles older than 12 months are open to all at the journal site (alternative location) |
URI: | http://hdl.handle.net/2289/4038 |
ISSN: | 0006-3495 1542-0086 (Online) |
Alternative Location: | http://arxiv.org/abs/1006.1503 http://dx.doi.org/10.1016/j.bpj.2010.10.027 |
Copyright: | 2010 Biophysical Society |
Appears in Collections: | Research Papers (SCM) |
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2010_Biophysical Journal_V99_p3571.pdf Restricted Access | Restricted Access | 747.05 kB | Adobe PDF | View/Open Request a copy |
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