Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/4038
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dc.contributor.authorFernandez, Pablo-
dc.contributor.authorPullarkat, Pramod A.-
dc.date.accessioned2011-04-08T09:30:14Z-
dc.date.available2011-04-08T09:30:14Z-
dc.date.issued2010-12-01-
dc.identifier.citationBiophysical Journal, 2010, Vol.99, p3571en
dc.identifier.issn0006-3495-
dc.identifier.issn1542-0086 (Online)-
dc.identifier.urihttp://hdl.handle.net/2289/4038-
dc.descriptionRestricted Access. Articles older than 12 months are open to all at the journal site (alternative location)en
dc.description.abstractWe present investigations on volume regulation and beading shape transitions in PC12 neurites conducted using a flow chamber technique By disrupting the cell cytoskeleton with specific drugs we investigate the role of its individual components in the volume regulation response We find that microtubule disruption increases both swelling rate and maximum volume attained but does not affect the ability of the neurite to recover its initial volume In addition investigation of axonal beading also known as pearling instability provides additional clues on the mechanical state of the neurite We conclude that volume recovery is driven by passive diffusion of osmolites and propose that the initial swelling phase is mechanically slowed down by microtubules Our experiments provide a framework to investigate the role of cytoskeletal mechanics in volume homeostasisen
dc.language.isoenen
dc.publisherElsevier for Biophysical Societyen
dc.relation.urihttp://arxiv.org/abs/1006.1503en
dc.relation.urihttp://dx.doi.org/10.1016/j.bpj.2010.10.027en
dc.rights2010 Biophysical Societyen
dc.subjectAtomic-force microscopyen
dc.subjectnerve growth-factoren
dc.subjectcell-volumeen
dc.subjectfibroblastsen
dc.subjectmicrotubulesen
dc.subjectpermeabilityen
dc.subjectorganizationen
dc.subjectkinetics wateren
dc.titleThe role of the cytoskeleton in volume regulation and beading transitions in PC12 neuritesen
dc.typeArticleen
Appears in Collections:Research Papers (SCM)

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