Investigation of oxidative-stress-induced axon beading using photo-oxidation

Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/8638

Title:	Investigation of oxidative-stress-induced axon beading using photo-oxidation
Authors:	Mehta, Deepak Joshi, Pooja Pullarkat, Pramod
Keywords:	axonal beading axonal degeneration oxidative stress photo-oxidation phototoxicity reactive oxygen species
Issue Date:	9-Jun-2025
Publisher:	FEBS Letters
Citation:	FEBS Letters, 2025, Vol. 599(17), p 2491-2503
Abstract:	Oxidative stress caused by excessive reactive oxygen species adversely affects cellular function. Here, we investigate axonal beading induced by the photo-oxidation of a membrane-based fluorescent dye. Our experiments show that, apart from using oxygen scavengers, chelation of free calcium, or stabilization of microtubules or actin filaments by pharmacological agents can mitigate oxidative-stress-induced beading. Furthermore, we take advantage of this light-induced oxidative stress to explore axonal responses to spatially confined perturbations. We demonstrate that low excitation causes localized, long-lasting axon beading, whereas high excitation leads to widespread degeneration and beading. Besides the results presented here, this light-based technique is a convenient tool for investigating local oxidative stress in tissues, such as brain slices or organoids.
Description:	Restricted Access.
URI:	http://hdl.handle.net/2289/8638
ISSN:	1873-3468
Alternative Location:	https://doi.org/10.1002/1873-3468.70074
Copyright:	2025 Federation of European Biochemical Societies
Appears in Collections:	Research Papers (SCM)

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Supplementary information (Investigation of oxidative stress .pdf Restricted Access	Restricted Access	802.24 kB	Adobe PDF	View/Open Request a copy