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Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/855
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dc.contributor.authorSinha, D.K.-
dc.contributor.authorBhattacharya, D.-
dc.contributor.authorBanerjee, Bidisha-
dc.contributor.authorHameed, F.M.-
dc.contributor.authorShivashankar, G.V.-
dc.date.accessioned2005-12-26T08:40:14Z-
dc.date.available2005-12-26T08:40:14Z-
dc.date.issued2004-07-25-
dc.identifier.citationCurrent Science, 2004, Vol. 87, p239-244.en
dc.identifier.issn0011-3891-
dc.identifier.urihttp://hdl.handle.net/2289/855-
dc.description.abstractWe have constructed a confocal fluorescence micro-scope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The metho-dology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence corre-lation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence ani-sotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression.en
dc.format.extent214487 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherIndian Academy of Sciences, Bangalore, India.en
dc.rightsIndian Academy of Sciences, Bangalore, India.en
dc.titleDevelopment of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysisen
dc.typeArticleen
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