Development of an optical tweezer combined with micromanipulation for DNA and protein  nanobioscience

Soni, G.V.; Hameed, F.M.; Roopa, T.; Shivashankar, G.V.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/824

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DC Field	Value	Language
dc.contributor.author	Soni, G.V.	-
dc.contributor.author	Hameed, F.M.	-
dc.contributor.author	Roopa, T.	-
dc.contributor.author	Shivashankar, G.V.	-
dc.date.accessioned	2005-12-26T05:58:38Z	-
dc.date.available	2005-12-26T05:58:38Z	-
dc.date.issued	2002-12-25	-
dc.identifier.citation	Current Science, 2002, Vol. 83, p1464-1470.	en
dc.identifier.issn	0011-3891	-
dc.identifier.uri	http://hdl.handle.net/2289/824	-
dc.description.abstract	We have constructed an optical tweezer using two lasers (830 nm and 1064 nm) combined with micropipette manipulation having sub-pN force sensitivity. Sample position is controlled within nanometer accuracy using XYZ piezo-electric stage. The position of the bead in the trap is monitored using single particle laser backscattering technique. The instrument is automated to operate in constant force, constant velocity or constant position measurement. We present data on single DNA force-extension, dynamics of DNA integration on membranes and optically trapped beadcell interactions. A quantitative analysis of single DNA and protein mechanics, assembly and dynamics opens up new possibilities in nanobioscience.	en
dc.format.extent	257481 bytes	-
dc.format.mimetype	application/pdf	-
dc.language.iso	en	en
dc.publisher	Indian Academy of Sciences, Bangalore, India.	en
dc.rights	Indian Academy of Sciences, Bangalore, India.	en
dc.title	Development of an optical tweezer combined with micromanipulation for DNA and protein nanobioscience	en
dc.type	Article	en
Appears in Collections:	Research Papers (TP)

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