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Title: Effect of pH on the phase behavior of DMPC bilayers
Authors: Chowdhury, Anindya
Sasidharan, Sreeja
Xavier, Pinchu
Viswanath, P.
Raghunathan, V.A.
Issue Date: Nov-2021
Citation: Biochimica et Biophysica Acta (BBA) - Biomembranes, 2021, Vol. 1863, p183695
Abstract: We have studied the effect of acidic pH on the phase behavior of the zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) using differential scanning calorimetry and x-ray scattering. Dispersions of DMPC in HCl solutions of pH = 4 and 3 behave identical to dispersions in water. The main transition temperature increases sharply and the pre-transition disappears at lower pH. An untilted gel phase is observed at pH = 2 and 1, in contrast to the tilted gel phase found at higher pH. The relatively large periodicity of the untilted gel phase, in comparison to that of the tilted gel phase occurring near neutral pH, clearly demonstrates the simultaneous charging and dehydration of the headgroups as the pH approaches the pK of the phosphate group. Headgroup dehydration at low pH also leads to the formation of DMPC crystallites and the inverted hexagonal phase at low and high temperatures, respectively, after a few days of incubation. These results show the significant effect of acidic pH on the phase behavior of zwitterionic lipids.
Description: Restricted Access.
ISSN: 0006-3002
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Copyright: 2021 Elsevier B.V.
Appears in Collections:Research Papers (SCM)

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