Please use this identifier to cite or link to this item:
http://hdl.handle.net/2289/7719| Title: | Measurement of Alcohol-Dependent Physiological Changes in Red Blood Cells Using Resistive Pulse Sensing |
| Authors: | Kaushik, Saurabh Mahadeva, Manohara Durai Murugan, K. Sundaramurthy, V. Soni, G.V. |
| Keywords: | size of red blood cells Electrical conductivity Manufacturing resistive pulse technique microspores |
| Issue Date: | 24-Dec-2020 |
| Publisher: | American Chemical Society |
| Citation: | ACS Sensors, 2020, Vol.5, p. 3892-3901 |
| Abstract: | Alcohol exposure has been postulated to adversely affect the physiology and function of the red blood cells (RBCs). The global pervasiveness of alcohol abuse, causing health issues and social problems, makes it imperative to resolve the physiological effects of alcohol on RBC physiology. Alcohol consumed recreationally or otherwise almost immediately alters cell physiology in ways that is subtle and still unresolved. In this paper, we introduce a high-resolution device for quantitative electrofluidic measurement of changes in RBC volume upon alcohol exposure. We present an exhaustive calibration of our device using model cells to measure and resolve volume changes down to 0.6 fL. We find an RBC shrinkage of 5.3% at 0.125% ethanol (the legal limit in the United States) and a shrinkage of 18.5% at 0.5% ethanol (the lethal limit) exposure. Further, we also measure the time dependence of cell volume shrinkage (upon alcohol exposure) and then recovery (upon alcohol removal) to quantify shrinkage and recovery of RBC volumes. This work presents the first direct quantification of temporal and concentration-dependent changes in red blood cell volume upon ethanol exposure. Our device presents a universally applicable high-resolution and high-throughput platform to measure changes in cell physiology under native and diseased conditions. |
| Description: | Restricted Access. |
| URI: | http://hdl.handle.net/2289/7719 |
| ISSN: | E-ISSN 2379-3694 |
| Alternative Location: | DOI: 10.1021/acssensors.0c01302 |
| Copyright: | 2020, American Chemical Society |
| Appears in Collections: | Research Papers (SCM) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2020_ACSsensorsVol.5,SI.pdf Restricted Access | 1.62 MB | Adobe PDF | View/Open Request a copy | |
| 2020_ACS Sensors_Vol.5, p3892−3901.pdf Restricted Access | Restricted Access | 2.33 MB | Adobe PDF | View/Open Request a copy |
| se0c01302_si_002.avi Restricted Access | 11.38 MB | VideoClip | View/Open Request a copy |
Items in RRI Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.