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Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/7356
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dc.contributor.authorSasidharan, Sreeja-
dc.contributor.authorRaghunathan, V.A.-
dc.contributor.author+4 Co-Authors-
dc.date.accessioned2019-11-19T05:40:40Z-
dc.date.available2019-11-19T05:40:40Z-
dc.date.issued2019-10-28-
dc.identifier.citationSoft Matter, 2019, Vol.15, p8129en_US
dc.identifier.issn1744-683X-
dc.identifier.issn1744-6848 (Online)-
dc.identifier.urihttp://hdl.handle.net/2289/7356-
dc.descriptionRestricted Access.en_US
dc.description.abstractInteraction between mononucleotides and lipid membranes is believed to have played an important role in the origin of life on Earth. Studies on mononucleotide-lipid systems hitherto have focused on the influence of the lipid environment on the organization of the mononucleotide molecules, and the effect of the latter on the confining medium has not been investigated in detail. We have probed the interaction of the mononucleotide, uridine 5′-monophosphate (UMP), and its disodium salt (UMPDSS) with fluid dimyristoylphosphatidylcholine (DMPC) membranes, using small-angle X-ray scattering (SAXS), cryogenic scanning electron microscopy (cryo-SEM) and computer simulations. UMP adsorbs and charges the lipid membrane, resulting in the formation of unilamellar vesicles in dilute solutions. Adsorption of UMP reduces the bilayer thickness of DMPC. UMPDSS has a much weaker effect on interbilayer interactions. These observations are in very good agreement with the results of an all-atom molecular dynamics simulation of these systems. In the presence of counterions, such as Na+, UMP forms small aggregates in water, which bind to the bilayer without significantly perturbing it. The phosphate moiety in the lipid headgroup is found to bind to the hydrogens from the sugar ring of UMP, while the choline group tends to bind to the two oxygens from the nucleotide base. These studies provide important insights into lipid–nucleotide interactions and the effect of the nucleotide on lipid membranes.en_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.urihttp://dx.doi.org/10.1039/C9SM01257Een_US
dc.rights2019 Royal Society of Chemistryen_US
dc.titleInteraction of the mononucleotide UMP with a fluid phospholipid bilayer†en_US
dc.typeArticleen_US
dc.additionalSupplementary information Availableen_US
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