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http://hdl.handle.net/2289/7053
Title: | Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells |
Authors: | Thottacherry, Joseph Jose Kumar, Amit Pullarkat, Pramod A. + 15 Co-Authors |
Issue Date: | 11-Oct-2018 |
Publisher: | Springer Nature |
Citation: | Nature Communications, 2017, Vol. 9, AN 4217 |
Abstract: | Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells. |
Description: | Open access |
URI: | http://hdl.handle.net/2289/7053 |
ISSN: | 2041-1723 (online) |
Alternative Location: | https://www.biorxiv.org/content/early/2017/10/25/201509 https://doi.org/10.1038/s41467-018-06738-5 |
Copyright: | 2018 Springer Nature |
Additional information: | Supplementary information available |
Appears in Collections: | Research Papers (SCM) |
Files in This Item:
File | Description | Size | Format | |
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2018_Nature Communications_Vol.9_p4217.pdf Restricted Access | Open Access | 4.32 MB | Adobe PDF | View/Open Request a copy |
2018_Nature Communications_Vol.9_p4217_Information support.pdf Restricted Access | Open Access | 10 MB | Adobe PDF | View/Open Request a copy |
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