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http://hdl.handle.net/2289/6909
Title: | Modeling cell-substrate de-adhesion dynamics under fluid shear |
Other Titles: | Maan, Renu; Rani, Garima; Menon, Gautam, |
Authors: | Renu, Maan Garima, Rani Gautam, Menon I Pullarkat, Pramod A. |
Keywords: | cell shearing mechanobiology adhesion strength cell detachment model cell de-adhesion |
Issue Date: | 9-May-2018 |
Publisher: | IOP Publishing |
Citation: | Physical Biology, 2018, Vol.15, P 046006 |
Abstract: | Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of ~500 pN associated to a single focal contact, and characteristic time-scales of s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis. |
Description: | Restricted Access. |
URI: | http://hdl.handle.net/2289/6909 |
ISSN: | 1478-3967 1478-3975 (Online) |
Alternative Location: | http://dx.doi.org/10.1088/1478-3975/aabc66 |
Copyright: | 2018 IOP Publishing Ltd |
Additional information: | Supplementary material is available |
Appears in Collections: | Research Papers (SCM) |
Files in This Item:
File | Description | Size | Format | |
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2018_Physical Biology_Vol.15_p046006.pdf Restricted Access | Restricted Access | 1.13 MB | Adobe PDF | View/Open Request a copy |
2018_Physical Biology_Vol.15_p046006_Supp Info.pdf Restricted Access | Restricted Access | 564.93 kB | Adobe PDF | View/Open Request a copy |
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