1. RRI Digital Repository
  2. 07. Theoretical Physics
  3. Research Papers (TP)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/5555
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTalwar, Shefali-
dc.contributor.authorKumar, Abhishek-
dc.contributor.authorRao, Madan-
dc.contributor.authorMenon, Gautam I.-
dc.contributor.authorShivashankar, G.V.-
dc.date.accessioned2013-04-24T06:39:37Z-
dc.date.available2013-04-24T06:39:37Z-
dc.date.issued2013-02-
dc.identifier.citationBiophysical Journal, 2013, Vol.104, p 553-564en
dc.identifier.issn0006-3495-
dc.identifier.issn1542-0086(Online)-
dc.identifier.urihttp://hdl.handle.net/2289/5555-
dc.descriptionRestricted Access. An open-access version is available at arXiv.org (one of the alternative locations)en
dc.description.abstractStem cells integrate signals from the microenvironment to generate lineage-specific gene expression programs upon differentiation. Undifferentiated cell nuclei are easily deformable, with an active transcriptome, whereas differentiated cells have stiffer nuclei and condensed chromatin. Chromatin organization in the stem cell state is known to be highly dynamic but quantitative characterizations of its plasticity are lacking. Using fluorescence imaging, we study the spatio-temporal dynamics of nuclear architecture and chromatin compaction in mouse embryonic stem (ES) cells and differentiated states. Individual ES cells exhibit a relatively narrow variation in chromatin compaction, whereas primary Mouse embryonic fibroblasts (PMEF) show broad distributions. However, spatial correlations in chromatin compaction exhibit an emergent length scale in PMEFs, although they are unstructured and longer ranged in ES cells. We provide evidence for correlated fluctuations with large amplitude and long intrinsic timescales, including an oscillatory component, in both chromatin compaction and nuclear area in ES cells. Such fluctuations are largely frozen in PMEF. The role of actin and Lamin A/C in modulating these fluctuations is described. A simple theoretical formulation reproduces the observed dynamics. Our results suggest that, in addition to nuclear plasticity, correlated spatio-temporal structural fluctuations of chromatin in undifferentiated cells characterize the stem cell state.en
dc.language.isoenen
dc.publisherBiophysical Societyen
dc.relation.urihttp://dx.doi.org/10.1016/j.bpj.2012.12.033en
dc.relation.urihttp://adsabs.harvard.edu/abs/2013BpJ...104..553Ten
dc.rights2013 Biophysical Societyen
dc.titleCorrelated Spatio-Temporal Fluctuations in Chromatin Compaction States Characterize Stem Cellsen
dc.typeArticleen
Appears in Collections:Research Papers (TP)

Files in This Item:
File Description SizeFormat 
2013_Biophysical Journal_V104_p553.pdf
  Restricted Access		Restricted Access2.27 MBAdobe PDFView/Open Request a copy
Show simple item record


Items in RRI Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.