Active remodeling of cortical Actin regulates spatiotemporal organization of cell surface molecules

Gowrishankar, Kripa; Ghosh, Subhasri; Saha, Suvrajit; Rumamol, C.; Mayor, Satyajit; Rao, Madan

Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/5093

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DC Field	Value	Language
dc.contributor.author	Gowrishankar, Kripa	-
dc.contributor.author	Ghosh, Subhasri	-
dc.contributor.author	Saha, Suvrajit	-
dc.contributor.author	Rumamol, C.	-
dc.contributor.author	Mayor, Satyajit	-
dc.contributor.author	Rao, Madan	-
dc.date.accessioned	2012-07-26T09:37:01Z	-
dc.date.available	2012-07-26T09:37:01Z	-
dc.date.issued	2012-06-08	-
dc.identifier.citation	Cell, 2012, Vol.149, p1353-1367	en
dc.identifier.issn	0092-8674	-
dc.identifier.uri	http://hdl.handle.net/2289/5093	-
dc.description	Restricted Access.	en
dc.description.abstract	Many lipid-tethered proteins and glycolipids exist as monomers and nanoclusters on the surface of living cells. The spatial distribution and dynamics of formation and breakup of nanoclusters does not reflect thermal and chemical equilibrium and is controlled by activeremodeling of the underlying corticalactin. We propose a model for nanoclustering based on active hydrodynamics, wherein cell surface molecules bound to dynamic actin are actively driven to form transient clusters. This consistently explains all of our experimental observations. Using FCS and TIRF microscopy, we provide evidence for the existence of short, dynamic, polymerizing actin filaments at the cortex, a key assumption of the theoretical framework. Our theory predicts that lipid-anchored proteins that interact with dynamic actin must exhibit anomalous concentration fluctuations, and a cell membrane protein capable of binding directly to actin can form nanoclusters. These we confirm experimentally, providing an active mechanism for molecular organization and its spatiotemporal regulation on the plasma membrane.	en
dc.language.iso	en	en
dc.publisher	Elsevier Inc.	en
dc.relation.uri	http://dx.doi.org/10.1016/j.cell.2012.05.008	en
dc.rights	2012 Elsevier Inc.	en
dc.title	Active remodeling of cortical Actin regulates spatiotemporal organization of cell surface molecules	en
dc.type	Article	en
Appears in Collections:	Research Papers (TP)

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