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|Title: ||Shape oscillations of non-adhering fibroblast cells|
|Authors: ||Salbreux, G.|
Pullarkat, Pramod A.
|Issue Date: ||2007 |
|Publisher: ||Institute of Physics|
|Citation: ||Physical Biology , 2007, Vol.4, p268–284|
|Abstract: ||We demonstrate that 3T3 fibroblast cells can exhibit periodic shape oscillations following a loss of cell-substrate adhesion. The oscillatory behavior can last many hours at a constant
frequency, and can be switched off and on using chemical agents. We show that the oscillation
frequency increases with increasing acto-myosin contractility. The oscillations also cease
when extracellular calcium is depleted or when a blocker of calcium channels is introduced.
We propose a theoretical description of the oscillations based on an instability of the cortical
actin layer. The cortical actin layer is described using the hydrodynamic theory of active gels.
We assume that calcium enters the cell via mechanically gated channels and that an increase of
the calcium density increases the acto-myosin contractility in the cortical layer. The theory
provides a stability diagram for the actin cortical layer showing an oscillatory instability and
gives a good description of the oscillation period. We also discuss the connections between
these oscillations and other oscillations observed after depolymerization of the microtubules
and with the formation of blebs.|
|Description: ||Restricted Access.|
|Copyright: ||2007 Institute of Physics|
|Appears in Collections:||Research Papers (SCM)|
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