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Title: Integrin adhesion drives the emergent polarization of active cytoskeletal stresses to pattern cell delamination
Authors: Meghanaa, C.
Ramdas, Nisha
Hameed, F.M.
Rao, Madan
Shivashankar, G.V.
Narasimha, Maithreyi
Keywords: cell extrusion
cell mechanics
tissue dynamics
Issue Date: 31-May-2011
Publisher: National Academy of Sciences
Citation: Proceedings of the National Academy of Sciences, 2011, Vol.108, p9107
Abstract: Tissue patterning relies on cellular reorganization through the interplay between signaling pathways and mechanical stresses. Their integration and spatiotemporal coordination remain poorly understood. Here we investigate the mechanisms driving the dynamics of cell delamination, diversely deployed to extrude dead cells or specify distinct cell fates. We show that a local mechanical stimulus (subcellular laser perturbation) releases cellular prestress and triggers cell delamination in the amnioserosa during Drosophila dorsal closure, which, like spontaneous delamination, results in the rearrangement of nearest neighbors around the delaminating cell into a rosette. We demonstrate that a sequence of “emergent cytoskeletal polarities” in the nearest neighbors (directed myosin flows, lamellipodial growth, polarized actomyosin collars, microtubule asters), triggered by the mechanical stimulus and dependent on integrin adhesion, generate active stresses that drive delamination. We interpret these patterns in the language of active gels as asters formed by active force dipoles involving surface and body stresses generated by each cell and liken delamination to mechanical yielding that ensues when these stresses exceed a threshold. We suggest that differential contributions of adhesion, cytoskeletal, and external stresses must underlie differences in spatial pattern.
Description: Restricted Access.
ISSN: 0027-8424
1091-6490 (Online)
Alternative Location:
Copyright: 2011 National Academy of Sciences
Appears in Collections:Research Papers (TP)

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