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Title: Probing the dynamic organization of transcription compartments and gene loci within the nucleus of living cells
Authors: Sinha, D.K.
Banerjee, Bidisha
Maharana, Shovamayee
Shivashankar, G.V.
Issue Date: Dec-2008
Publisher: Biophysical Society/Highwire Press
Citation: Biophysical Journal, 2008, Vol.95, p5432
Abstract: The three-dimensional organization of nuclear compartments within living cells determines genome function and yet their underlying self-organizing principles are unclear. We visualize in real-time transcriptionally active compartments (TCs) by the transient enrichment of fluorescently-labeled uridine 5-triphosphate molecules within living cells. These TCs partially colocalize with active RNA-Pol II in the cell nucleus. Fluorescence anisotropy maps of chromatin compaction evidences a more open chromatin structure at the TCs. Using live-cell timelapse imaging, heterogeneity in the dynamic behavior of TCs has been revealed which falls into three distinct classes: subdiffusive, super-diffusive, and normal diffusive behavior. In contrast, the mobility of a candidate gene locus, either in the repressed or activated state, undergoes a differential restricted motion that is coupled to TC movement. Further TC dynamics is directly affected by small molecule chromatin structure modulators and adenosine triphosphate depletion. This heterogeneous behavior in TC dynamics within living cells could provide an interesting paradigm to explore the spatiotemporal dimension to gene transcription control.
Description: Restricted Access. Articles older than 12 months are open to all at the journal site (alternative location)
ISSN: E-ISSN: 1542-0086
P-ISSN: 0006-3495
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Copyright: 208 The Biophysical Society
Appears in Collections:Research Papers (SCM)

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