Gold-nanoparticle-assisted laser perturbation of chromatin assembly reveals unusual aspects of nuclear architecture within living cells

Mazumder, Aprotim; Shivashankar, G.V.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/3667

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DC Field	Value	Language
dc.contributor.author	Mazumder, Aprotim	-
dc.contributor.author	Shivashankar, G.V.	-
dc.date.accessioned	2008-11-28T11:16:30Z	-
dc.date.available	2008-11-28T11:16:30Z	-
dc.date.issued	2007-09	-
dc.identifier.citation	Biophysical Journal, 2007, Vol.93, p2209	en
dc.identifier.issn	E-ISSN: 1542-0086	-
dc.identifier.issn	P-ISSN: 0006-3495	-
dc.identifier.uri	http://hdl.handle.net/2289/3667	-
dc.description	Restricted Access. Articles older than 12 months are open to all at the journal site (alternative location)	en
dc.description.abstract	Chromatin organization within the nucleus is a vital regulator of genome function, yet its mechanical coupling to the nuclear architecture has remained elusive. To directly investigate this coupling, we locally modulated chromatin structure in living cells using nanoparticle-based laser perturbation. Unusual differences in the response of the cell nucleus were observed depending on the nuclear region that was perturbed—the heterochromatin, the euchromatin, and the nuclear envelope. This response varied under different conditions of cellular perturbations such as ATP depletion, apoptosis, and inhibition of histone deacetylases. Our studies implicate heterochromatin organization in imparting mechanical stability to the cell nucleus and suggest that nuclear size and shape are the result of interplay between nuclear and cytoplasmic anchors.	en
dc.language.iso	en	en
dc.publisher	Biophysical Society/Highwire Press	en
dc.relation.uri	http://dx.doi.org/10.1529/biophysj.106.102202	en
dc.rights	2007 The Biophysical Society	en
dc.title	Gold-nanoparticle-assisted laser perturbation of chromatin assembly reveals unusual aspects of nuclear architecture within living cells	en
dc.type	Article	en
Appears in Collections:	Research Papers (SCM)

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