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Title: Kinetic measurement of ribosome motor stalling force
Authors: Sinha, D.K.
Bhalla, U.S.
Shivashankar, G.V.
Issue Date: 15-Nov-2004
Publisher: The American Institute of Physics
Citation: Applied Physics Letters, 2004, Vol.85, p4789-4791.
Abstract: We measure the ribosome motor stalling forces to unzip mRNA polymers during gene expression. An approach of using the changes in the reaction rate constants to determine the molecular motor forces is presented. Specific antisense DNA oligomers complementary to mRNA templates are used as kinetic barriers for estimating the ribosome forces using real time bioluminescence detection of luciferase gene expression. The rate constants are determined by comparing the experimental data with numerical simulation of gene expression to deduce the ribosome force (26.5±1 pN) required to unzip mRNA polymers. Understanding the forces generated by the ribosome may also enable the construction of information-based artificial nanoscale machines.
ISSN: 0003-6951
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Copyright: (2004) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.
Appears in Collections:Research Papers (SCM)

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