Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/1246
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSinha, D.K.-
dc.contributor.authorSastry, Srikanth-
dc.contributor.authorShivashankar, G.V.-
dc.date.accessioned2006-05-16T09:41:13Z-
dc.date.available2006-05-16T09:41:13Z-
dc.date.issued2006-03-06-
dc.identifier.citationApplied Physics Letters, 2006, Vol.88, p103901-103903en
dc.identifier.issn0003-6951-
dc.identifier.urihttp://hdl.handle.net/2289/1246-
dc.descriptionOpen Access-
dc.description.abstractIn this letter we describe a method to probe biomolecular conformations and their dynamics at the single molecule level. We show, using fluorescence anisotropy based methods, that the hydrodynamic volume of biomolecules captures the intrinsic heterogeneity within a population. Population distributions of conformations and their dynamics are studied by making anisotropy measurements on one molecule at a time within a confocal volume. The mean anisotropy of mRNA is lowered on addition of salt while the spread remains the same. The intrinsic heterogeneity is revealed when conformational transitions are frozen, resulting in a drastic increase in the spread of the anisotropy. These studies reveal that mRNA samples a broad range of conformations.en
dc.format.extent118878 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherThe American Institute of Physicsen
dc.relation.urihttp://link.aip.org/link/?apl/88/103901en
dc.rights(2006)American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.en
dc.titleProbing messenger RNA conformational heterogeneity using single-molecule fluorescence anisotropyen
dc.typeArticleen
Appears in Collections:Research Papers (TP)

Files in This Item:
File Description SizeFormat 
2006 APL V88 p103901.pdf3p.116.09 kBAdobe PDFView/Open


Items in RRI Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.