Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis
| dc.contributor.author | Sinha, D K | |
| dc.contributor.author | Bhattacharya, D | |
| dc.contributor.author | Banerjee, Bidisha | |
| dc.contributor.author | Hameed, F M | |
| dc.contributor.author | Shivashankar, G V | |
| dc.date.accessioned | 2005-12-26T08:40:14Z | |
| dc.date.available | 2005-12-26T08:40:14Z | |
| dc.date.issued | 2004-07-25 | |
| dc.description.abstract | We have constructed a confocal fluorescence micro-scope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The metho-dology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence corre-lation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence ani-sotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression. | en |
| dc.format.extent | 214487 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Current Science, 2004, Vol. 87, p239-244. | en |
| dc.identifier.issn | 0011-3891 | |
| dc.identifier.uri | http://hdl.handle.net/2289/855 | |
| dc.language.iso | en | en |
| dc.publisher | Indian Academy of Sciences, Bangalore, India. | en |
| dc.rights | Indian Academy of Sciences, Bangalore, India. | en |
| dc.title | Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis | en |
| dc.type | Article | en |