Phase behavior of two-component lipid membranes: Theory and experiments

Abstract

The structure of the ripple phase of phospholipid membranes remains poorly understood in spite of a large number of theoretical studies, with many experimentally established structural features of this phase unaccounted for. In this article we present a phenomenological theory of phase transitions in single- and two-component achiral lipid membranes in terms of two coupled order parameters: a scalar order parameter describing lipid chain melting, and a vector order parameter describing the tilt of the hydrocarbon chains below the chain-melting transition. This model reproduces all the salient structural features of the ripple phase, providing a unified description of the phase diagram and microstructure. In addition, it predicts a variant of this phase that does not seem to have been experimentally observed. Using this model we have calculated generic phase diagrams of two-component membranes. We have also determined the phase diagram of a two-component lipid membrane from x-ray diffraction studies on aligned multilayers. This phase diagram is found to be in good agreement with that calculated from the model.