1. RRI Digital Repository
  2. 06. Soft Condensed Matter
  3. Research Papers (SCM)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/8640
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBodas, Shivani-
dc.contributor.authorMishra, Ashish-
dc.contributor.authorPullarkat, Pramod-
dc.contributor.authorGhose, Aurnab-
dc.date.accessioned2026-02-11T08:51:10Z-
dc.date.available2026-02-11T08:51:10Z-
dc.date.issued2025-07-28-
dc.identifier.citationelife, 2025, Vol. 1, Article No.107797en_US
dc.identifier.issn2050-084X-
dc.identifier.urihttp://hdl.handle.net/2289/8640-
dc.descriptionOpen Access.en_US
dc.description.abstractThe axonal membrane-associated periodic skeleton (MPS), consisting of F-actin rings crosslinked by spectrin heterotetramers, is ubiquitous and critical for neuronal function and homoeostasis. However, the initiation and early development of the axonal MPS are poorly understood. Using superresolution imaging, we show that βII-spectrin is recruited early to the axonal cortex, followed by progressive establishment of long-range periodic order. Microtubule dynamics are essential for MPS formation in the early stages, but transition to a passive stabilising role in mature axons. We show that the early subplasmalemmal recruitment of βII-spectrin is dependent on cortical actin but not on actomyosin contractility, and active nucleation of F-actin is required in early development but is dispensable for the mature MPS. Using a βII-spectrin knockout model, we demonstrate that the actin-binding and lipid-interacting domains of βII-spectrin are critical for its subplasmalemmal confinement and, subsequently, MPS maturation. These findings highlight stage-specific cytoskeletal remodelling underlying MPS development and advance our understanding of axonal subcellular architecture.en_US
dc.language.isoenen_US
dc.publishereLife Sciences Publications Ltden_US
dc.relation.urihttps://www.biorxiv.org/content/10.1101/2025.02.19.639207v2en_US
dc.relation.urihttps://doi.org/10.1101/2025.02.19.639207en_US
dc.rightseLife Sciences Publications Ltden_US
dc.titleDevelopment of the axonal βII-spectrin periodic skeleton requires active cytoskeletal remodellingen_US
dc.typeArticleen_US
dc.additionalThis study examines how the neuronal cytoskeleton contributes to the formation of the axonal membrane-associated periodic skeleton (MPS) in embryonic dorsal root ganglia (DRG) neurons, using STED imaging. Conclusions are supported by convincing methods, data, and analyses. This useful work confirms previous data and improves our understanding of the roles of microtubules and actin dynamics in the chronological recruitment of MPS components.en_US
Appears in Collections:Research Papers (SCM)

Files in This Item:
File Description SizeFormat 
2025_elife-Vol.1_Article No.107797.pdf
  Restricted Access		Open Access2.06 MBAdobe PDFView/Open Request a copy
Show simple item record


Items in RRI Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.