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http://hdl.handle.net/2289/855| Title: | Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis |
| Authors: | Sinha, D.K. Bhattacharya, D. Banerjee, Bidisha Hameed, F.M. Shivashankar, G.V. |
| Issue Date: | 25-Jul-2004 |
| Publisher: | Indian Academy of Sciences, Bangalore, India. |
| Citation: | Current Science, 2004, Vol. 87, p239-244. |
| Abstract: | We have constructed a confocal fluorescence micro-scope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The metho-dology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence corre-lation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence ani-sotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression. |
| URI: | http://hdl.handle.net/2289/855 |
| ISSN: | 0011-3891 |
| Copyright: | Indian Academy of Sciences, Bangalore, India. |
| Appears in Collections: | Research Papers (TP) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2004 CS 87 p239.pdf | 6p. | 209.46 kB | Adobe PDF | View/Open |
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