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http://hdl.handle.net/2289/8398Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kumar, Prateesh V.C. | - |
| dc.contributor.author | Pullarkat, Pramod | - |
| dc.date.accessioned | 2025-05-17T10:58:18Z | - |
| dc.date.available | 2025-05-17T10:58:18Z | - |
| dc.date.issued | 2025-04-24 | - |
| dc.identifier.citation | Neuroinformatics, 2025, Vol. 23, Article No.: 30 | en_US |
| dc.identifier.uri | http://hdl.handle.net/2289/8398 | - |
| dc.description | Restricted Access | en_US |
| dc.description.abstract | Axonal beading is a key morphological indicator of axonal degeneration, which plays a significant role in various neurodegenerative diseases and drug-induced neuropathies. Quantification of axonal susceptibility to beading using neuronal cell culture can be used as a facile assay to evaluate induced degenerative conditions, and thus aid in understanding mechanisms of beading and in drug development. Manual analysis of axonal beading for large datasets is labor-intensive and prone to subjectivity, limiting the reproducibility of results. To address these challenges, we developed a semi-automated Python-based tool to track axonal beading in time-lapse microscopy images. The software significantly reduces human effort by detecting the onset of axonal swelling. Our method is based on classical image processing techniques rather than an AI approach. This provides interpretable results while allowing the extraction of additional quantitative data, such as bead density, coarsening dynamics, and morphological changes over time. Comparison of results obtained through human analysis and the software shows strong agreement. The code can be easily extended to analyze diameter information of ridge-like structures in branched networks of rivers, road networks, blood vessels, etc. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Springer | en_US |
| dc.relation.uri | https://doi.org/10.1007/s12021-025-09726-5 | en_US |
| dc.rights | 2025 The Author(s) | en_US |
| dc.subject | Shape Analysis | en_US |
| dc.subject | Neural Patterning | en_US |
| dc.subject | Microscopy | en_US |
| dc.subject | Barrel Cortex | en_US |
| dc.subject | Axon and dendritic guidance | en_US |
| dc.subject | Automated Pattern Recognition | en_US |
| dc.title | Semi-automated Analysis of Beading in Degenerating Axons | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | Research Papers (SCM) | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2025_Neuroinformatics_Vol.23_Article No.30.pdf Restricted Access | Restricted Access | 1.23 MB | Adobe PDF | View/Open Request a copy |
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