1. RRI Digital Repository
  2. 06. Soft Condensed Matter
  3. Research Papers (SCM)
Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/6909
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRenu, Maan-
dc.contributor.authorGarima, Rani-
dc.contributor.authorGautam, Menon I-
dc.contributor.authorPullarkat, Pramod A.-
dc.date.accessioned2018-05-21T15:24:17Z-
dc.date.available2018-05-21T15:24:17Z-
dc.date.issued2018-05-09-
dc.identifier.citationPhysical Biology, 2018, Vol.15, P 046006en_US
dc.identifier.issn1478-3967-
dc.identifier.issn1478-3975 (Online)-
dc.identifier.urihttp://hdl.handle.net/2289/6909-
dc.descriptionRestricted Access.en_US
dc.description.abstractChanges in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of  ~500 pN associated to a single focal contact, and characteristic time-scales of s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.en_US
dc.language.isoenen_US
dc.publisherIOP Publishingen_US
dc.relation.urihttp://dx.doi.org/10.1088/1478-3975/aabc66en_US
dc.rights2018 IOP Publishing Ltden_US
dc.subjectcell shearingen_US
dc.subjectmechanobiologyen_US
dc.subjectadhesion strengthen_US
dc.subjectcell detachment modelen_US
dc.subjectcell de-adhesionen_US
dc.titleModeling cell-substrate de-adhesion dynamics under fluid shearen_US
dc.title.alternativeMaan, Renu; Rani, Garima; Menon, Gautam,en_US
dc.typeArticleen_US
dc.additionalSupplementary material is availableen_US
Appears in Collections:Research Papers (SCM)

Files in This Item:
File Description SizeFormat 
2018_Physical Biology_Vol.15_p046006.pdf
  Restricted Access		Restricted Access1.13 MBAdobe PDFView/Open Request a copy
2018_Physical Biology_Vol.15_p046006_Supp Info.pdf
  Restricted Access		Restricted Access564.93 kBAdobe PDFView/Open Request a copy
Show simple item record


Items in RRI Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.