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Please use this identifier to cite or link to this item: http://hdl.handle.net/2289/4062
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dc.contributor.authorMeghanaa, C.-
dc.contributor.authorRamdas, Nisha-
dc.contributor.authorHameed, F.M.-
dc.contributor.authorRao, Madan-
dc.contributor.authorShivashankar, G.V.-
dc.contributor.authorNarasimha, Maithreyi-
dc.date.accessioned2011-08-05T10:44:00Z-
dc.date.available2011-08-05T10:44:00Z-
dc.date.issued2011-05-31-
dc.identifier.citationProceedings of the National Academy of Sciences, 2011, Vol.108, p9107en
dc.identifier.issn0027-8424-
dc.identifier.issn1091-6490 (Online)-
dc.identifier.urihttp://hdl.handle.net/2289/4062-
dc.descriptionRestricted Access.en
dc.description.abstractTissue patterning relies on cellular reorganization through the interplay between signaling pathways and mechanical stresses. Their integration and spatiotemporal coordination remain poorly understood. Here we investigate the mechanisms driving the dynamics of cell delamination, diversely deployed to extrude dead cells or specify distinct cell fates. We show that a local mechanical stimulus (subcellular laser perturbation) releases cellular prestress and triggers cell delamination in the amnioserosa during Drosophila dorsal closure, which, like spontaneous delamination, results in the rearrangement of nearest neighbors around the delaminating cell into a rosette. We demonstrate that a sequence of “emergent cytoskeletal polarities” in the nearest neighbors (directed myosin flows, lamellipodial growth, polarized actomyosin collars, microtubule asters), triggered by the mechanical stimulus and dependent on integrin adhesion, generate active stresses that drive delamination. We interpret these patterns in the language of active gels as asters formed by active force dipoles involving surface and body stresses generated by each cell and liken delamination to mechanical yielding that ensues when these stresses exceed a threshold. We suggest that differential contributions of adhesion, cytoskeletal, and external stresses must underlie differences in spatial pattern.en
dc.language.isoenen
dc.publisherNational Academy of Sciencesen
dc.relation.urihttp://dx.doi.org/10.1073/pnas.1018652108en
dc.rights2011 National Academy of Sciencesen
dc.subjectcell extrusionen
dc.subjectcell mechanicsen
dc.subjecttissue dynamicsen
dc.subjectwound-healingen
dc.titleIntegrin adhesion drives the emergent polarization of active cytoskeletal stresses to pattern cell delaminationen
dc.typeArticleen
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